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Automated HPLC technique for determining diltiazem and its three main metabolites in serum
Summary
Type:
HPLC
Application #:
104850
Catalog#:
720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection:
UV, 240nm
Conditions:
eluent: 0.005M heptanesulphonic acid - acetonitrile - methanol - glacial acetic acid - triethylamine (60:35:5:5.5:0.75) flow rate 1.5ml/min
Authors:
Leneveu A
Source:
J Liquid Chromatogr 14 (1991) 3519 - 3530
Details
Legend: Fig. 1. Chromatograms of (C.1) blank serum, (C.2) human standard spiked with A) 20ng/ml M2 B) 20ng/ml M1 C) 20ng/ml MA D) 100ng/ml diltiazem (C.3) sample from a volunteer after a single 120mg o
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